Fig 10.

Sub-cellular localization of diphosphorylated regulatory light chain (RLC) in hUM. Immunofluorescent staining of hUM. (A–C) Filamentous actin (F-actin) stained with rhodamine-phalloidin (red). (D–F) Identical fields corresponding to panels A–C showing staining with a secondary Ab conjugated to Alexa-Fluor 488 (green). In panel D, the primary Ab has been omitted (secondary Ab only). In panels E and F, the anti-ppRLC Ab was used. (G) and (H) Merged images after combining panels B and E, or C and F respectively. Panels B, E and G correspond to vehicle (V) treatment, and panels C, F and H to cells stimulated with OT (100 nM). In all panels, nuclei are stained with DAPI (blue). Images are shown at 400× magnification. White bars represent 25 micrometres.