Fig 5.

Desynchronized [Ca²⁺]_i transients elicit contractions of alternating amplitude. (A) Example of alternating isometric force (upper trace) and its first derivative (lover trace) recorded from a strip in an organ bath experiment (typical of 23 recordings from 23 different samples). (B) Multi-cell [Ca²⁺]_i transients showing no synchronization (typical of five other slices from five different samples). Red and blue traces correspond to ROI positioned over two different bundles. Note that larger contractions occurred when [Ca²⁺]_i transients in different bundles coincided (marked by asterisks). (C) Single-cell ROIs positioned over two different cells in one bundle (red and orange traces) and two more cells in another bundle (blue and violet traces) in a slice with desynchronized bundles. Note that the [Ca²⁺]_i transients in individual cells within each bundle remained synchronized.