Fig 3.
mag-1-silencing defects metastasis of mouse breast cancer EMT6 cells in vivo. The EMT6/mag-1 KD cell strains, mock control and scramble-shRNA control (non-silencer) cells were inoculated to the mammary fat pad of female BALB/c mice respectively. Mice were killed when tumours reached a mean volume of 8 cm3 and the mice exhibited a symptom of cachexia. (A) Photographs of gross and histological representative tumour metastases within lungs arising from EMT6/mag-1 KD, scramble control and mock control cells. Scale bars represent 50 μm. (B) Total weight of tumour mass was measured after mice were dissected. (C) The numbers of metastatic foci within the histological specimen of lungs in each group observed and counted under light microscope. (D) Metastatic frequency following allograft injection of breast cancer cells. In all studies, statistical significance was determined using One-way anova with non-parametric analysis comparing data points to the scramble or mock control. Bars show the standard error of the mean.*P < 0.05; **P < 0.01. (Scale bar: 200 μM).