Fig 7.

Effect of mag-1 on tumour metastasis was significantly interrupted by mTOR inhibitor, rapamycin in vitro. (A) Effect of rapamycin on cell migration of PLA801C/mag-1 and mock control cells. Stably transfected PLA801C/mag-1 cells were treated or left untreated with 20 nM rapamycin for 12 hrs, and then subjected to wound healing assay. Representative photographs of wound closure monitored under microscope are shown and the wound healing rate was calculated as indicated in experimental procedures. (B) Effect of rapamycin on adhesion of PLA801C/mag-1 and mock control cells to Matrigel. Cells were pretreated or not by 20 nM rapamycin for 24 hrs, and then plated in the microwells coated with Matrigel. The detailed method is as mentioned previously. (C) Effect of rapamycin on cell invasion through Matrigel-coated transwell. Cells were pretreated or not by 20 nM rapamycin for 24 hrs, and then plated in the Matrigel-coated upper microwells of transwell. Each assay was performed thrice in triplicate. Bars show the standard error of the mean. Statistical significance was determined using One-way anova with non-parametric analysis comparing data points to the scramble or mock control. **P < 0.01; ***P < 0.001.