Figure 7.
Effect of pretreatment with Nrf2 inducers on BDE-47-stimulated IL-6 release from HTR-8/SVneo cells. A) Cells were pretreated with tert-butyl hydroquinone (tBHQ) for 1 h prior to subsequent incubation without or with 20 μM BDE-47 for 24 h. B) Cells were pretreated with sulforaphane for 24 h prior to subsequent incubation without or with 20 μM BDE-47 for 24 h. IL-6 levels were quantified using ELISA. Bars represent means±SEM (n=3 experiments). Each experiment was performed in triplicate. *P<0.05, significantly different compared to control (0 μM tBHQ or 0 μM sulforaphane with no BDE-47 treatment). #P<0.05, significantly different from each other. &P<0.05, significantly decreased compared with 0 μM and 5 μM sulforaphane pretreatment with BDE-47 exposure.