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. Author manuscript; available in PMC: 2016 Apr 1.
Published in final edited form as: J Neurochem. 2015 Feb 8;133(2):174–186. doi: 10.1111/jnc.13029

Figure 4. VPA induces AIF cleavage to a 57 kDa (tAIF) band and promotes its nuclear translocation.

Figure 4

(A) Neuronally differentiated PC12 cells were differentiated and mock- or VPA (1mM)-treated alone or in combination with PD as in Fig. 1B and whole cell extracts collected 72 hrs post-treatment were immunoblotted with AIF antibody. Data were quantified using densitometric scanning and are expressed as densitometric units ± SE. The levels of the 57kDa (tAIF) band were increased by VPA treatment and restored to those in mock-treated cells by PD treatment. (B) Neuronally differentiated PC12 and PC47 cells were mock- or VPA-treated as in Fig. 1 and cell extracts obtained 72 hrs post treatment were fractionated into nuclear (N) and cytoplasmic (C) fractions as described in MM. The fractions were immunoblotted with AIF antibody and the blots were stripped and re-probed with antibody to β-actin. Data were quantified by densitometric scanning and the results are expressed as densitometric units ± SE. The levels of the 57kDa tAIF band were significantly increased in both the cytoplasmic and nuclear fractions from the VPA-treated PC12, but not PC47 cells. (*P<0.001 by one-way ANOVA).