Fig. 6.

Polyubiquitin aggregates, lysosomal activity, and LC3-II expression in the muscle of young (2-month-old) and old (5-month-old) N. rachovii. a The expression of polyubiquitin aggregates in the muscle of young and old N. rachovii was analyzed through western blotting. Tubulin served as the loading control. b Lysosomal activity. The bars represent the mean ± SEM. c LC3-II expression was analyzed through western blotting. Tubulin served as the loading control. d LC3-II expression levels were normalized to that of young N. rachovii. The results are shown as percentages and represent the mean ± SEM. Asterisks indicate statistical significance determined by two-sample t tests (*P ≤ 0.05, **P ≤ 0.01)