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. Author manuscript; available in PMC: 2015 Apr 12.
Published in final edited form as: Environ Toxicol Chem. 2014 Dec;33(12):2767–2774. doi: 10.1002/etc.2738

Figure 1.

Figure 1

Average mortality, malformation rate, and pericardial area of 96–h postfertilization zebrafish embryos (n = 3, 10 embryos in each vial) dosed with 5% porewater with different pretreatment methods: (a) control with high-performance liquid chromatography (HPLC)–grade water, (b) raw porewater, (c) filtered porewater using a 0.7-μm glass fiber filter, (d) porewater after ultracentrifugation at 287 472 × g, (e) porewater after slow-speed centrifugation at 1000 ×g, (f) porewater after flocculation with alum, (g) porewater from overnight resuspension of settled particles after flocculation by alum. Error bar represents standard error. *Significant difference with the HPLC-grade water control.