Table 1.
The qPCR and RPKM results of the examined genes. We used qPCR to examine the expressions of nine overlapped genes and three nonoverlapped genes. EEF1A1 and HPRT1 are two commonly used internal control genes, while TMEM66 is the new internal control gene in this study. RPKM_SS and RPKM_NSS denote the RPKM values determined by the SS and NSS protocols, respectively. Ct1, Ct2, and Ct3 denote the Ct values from three independent qPCR experiments.
| Gene | RPKM_SS | RPKM_NSS | Ct1 | Ct2 | Ct3 |
|---|---|---|---|---|---|
| AMDHD2 | 3.58544 | 8.16441 | 26.30301 | 26.51864 | 26.41083 |
| ATP6V1C2 | 2.31219 | 32.661 | 32.18005 | 31.57895 | 31.8795 |
| COX11 | 23.49104 | 32.56102 | 21.27819 | 21.27837 | 21.27828 |
| DALRD3 | 5.24003 | 17.97184 | 23.26547 | 23.24969 | 23.25758 |
| MXD3 | 1.509837 | 8.58014 | 36.06333 | 35.29247 | 35.6779 |
| POLR2I | 8.01706 | 25.9707 | 19.39868 | 19.42806 | 19.41337 |
| SDR39U1 | 6.96971 | 20.5453 | 23.02526 | 23.05474 | 23.04 |
| SYNC | 1.306066 | 5.69769 | 28.14738 | 28.05567 | 28.10153 |
| TMOD1 | 1.30033 | 4.32226 | 27.98307 | 27.84805 | 27.91556 |
| EEF1A1 | 2248.53 | 2270.11 | 13.11139 | 13.15321 | 13.13354 |
| HPRT1 | 28.0151 | 28.3254 | 20.88358 | 21.07696 | 20.98027 |
| TMEM66 | 126.355 | 126.437 | 18.85524 | 18.85882 | 18.85703 |