Table 1.
Results of prediction software to determine whether proteins are transmembrane, secretory, or GPI-anchored. Transmembrane proteins were determined using the TMHMM server on the CBS Prediction Server with a posterior probability >0.8 and no N-terminus prediction on a transmembrane protein. N-terminus signaling peptides were elucidated using TargetP on the CBS Prediction Server, with a specificity >0.7 indicating a high probability of a N-terminus signaling peptide. GPI-SOM was used to predict C-terminus cleavage and anchoring sites. If a protein was not transmembrane and has both N-terminus and C-terminus it was predicted to be a GPI-anchored protein. If it had only a N-terminus prediction, it was classified as secretory protein. If it did not fit any category it was classified as a cytosolic-like protein.
|
Daphnia CA isoform criteria -> |
Transmembrane >0.8 and no N-terminus |
N-Terminus >0.7 specificity |
C-Terminus Most probable |
Prediction |
|---|---|---|---|---|
| CA5 | No | Yes | Yes | GPI-anchored |
| CA6A | No | Yes | Not | Secretory |
| CA6B | No | Yes | Not | Secretory |
| CA6C | No | Yes | Not | Secretory |
| CA6D | No | Yes | Not | Secretory |
| CA6E | No | 0.178 | Not | Cytosolic-like |
| CA6F | No | Yes | Yes | GPI-anchored |
| CA6G | No | Yes | Not | Secretory |
| CA6H | No | Yes | Not | Secretory |
| CA7A | No | Yes | Not | Secretory |
| CA7B | No | Yes | Not | Secretory |
| CA7C | No | Yes | Not | Secretory |
| CA7D | No | Yes | Not | Secretory |
| CA7E | No | Yes | Not | Secretory |
| CA7F | No | Yes | Not | Secretory |
| CA7G | No | Yes | Not | Secretory |
| CA7H | No | Yes | Yes | GPI-anchored |
| CA7I | No | Yes | Not | Secretory |
| CA7J | No | Yes | Not | Secretory |
| CA7K | No | Yes | Yes | GPI-anchored |
| CA7L | No | Yes | Not | Secretory |
| CA7M | No | Yes | Not | Secretory |
| CA7N | No | Yes | Not | Secretory |
| CA7O | No | Yes | Yes | GPI-anchored |
| CA7P | No | Yes | Not | Secretory |
| CA7Q | No | 0.288 | Not | Cytosolic-like |