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. Author manuscript; available in PMC: 2016 Apr 7.
Published in final edited form as: Structure. 2015 Mar 5;23(4):677–687. doi: 10.1016/j.str.2015.01.018

Figure 4. s9Nrp2B forms a disulfide-linked dimer.

Figure 4

(A) Domain organization of Nrp2 and the protein fragment utilized for our studies, s9Nrp2B. (B) Western blot analysis of Hgh-tagged s9Nrp2 and s9Nrp2B expressed in CHO cells. (C) Non-reducing and reducing SDS-PAGE analysis of Nrp2-b1 and s9Nrp2B. (D) The oligomeric state of s9Nrp2B (black line) was analyzed by size-exclusion chromatography. Nrp2-b1 was run as a reference (grey line).