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Effect of MN58b on ChoK activity in tumor cells. Autoradiography of TLC-separated 14C-choline containing metabolites (A) allows for the quantification of 14C-PC and demonstrates a dose-dependent reduction of choline phosphorylation in response to MN58b treatment. The graph (B) shows a dose dependent reduction of ChoK activity in the F98, 9L and 9L-EGFRviii cell lines. Error bars represent ± SEM of 3 separate experiments.
