Figure 2.
Sulforaphane does not inhibit HDAC activity under serum-depleted conditions. (A) Sub-confluent HaCaT cells were serum starved for 24 hr prior to addition of vehicle (AcN) or the indicated drugs, and nuclear proteins were extracted 8 hr later for the fluorometric HDAC activity assay. (B) HaCaT and HCT116 cells were serum starved for 24 hr prior to addition of vehicle or drugs (AcN, SF or Iso-9) and nuclear extracts were harvested 48 hr later for the same assay. VPA, TSA or water control were added 5 hr prior to harvest. Results representative of n = 3.