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Effect of elution volume. Elution buffer, 10 mM NH4HCO3 (pH=8); sequential elution volumes: (A) 60 nL, (B) 100 nL, (C) 140 nL, (D) 180 nL. Other experimental conditions the same as figure 2. Selected ion elecropherograms generated at m/z = 523.775.
