Table 1. Histopathological analysis of DSB-induced mouse liver.
| Pathology | Young | AdV | 1 mo post DSB | 2 mo post DSB | Old |
|---|---|---|---|---|---|
| Karyomegaly | 1.62±0.38 | 2.27±0.14 | 3.00±0* | 2.25±0.25 | 4.00±0** |
| Intranuclear inclusions | 0±0 | 0.09±0.10 | 0.38±0.13# | 0.38±0.13# | 3.00±0** |
| Lobular infiltrates | 0.38±0.24 | 0.81±0.26 | 1.63±0.13* | 1.38±0.24 | 3.67±0.33** |
| Portal infiltrates | 1.12±0.12 | 0.90±0.09 | 1.25±0.15* | 1.25±0.25 | 2.33±0.66* |
| EMH | 0±0 | 0±0 | 0.75±0.25* | 0.25±0.25 | 2.33±0.66* |
| Lipofuscin | 0.37±0.12 | 0.20±0.13 | 0.25±0.14 | 0±0 | 2.0±0.29* |
DSB, double-strand break; EMH, extramedullary haematopoiesis; mo, months.
Mice were killed and the livers were harvested at 1 mo or 2 mo post DSB induction. Livers were also harvested from untreated young mice (young), young SacI AdV injected mice without DOX (AdV) 1 and 2 months after injection and 28-month-old untreated aged controls (old). Liver sections were stained with haemotoxylin and eosin and assessed blinded for pathological characteristics of aging. Pathology was scored on a scale of 0–5, with 0 having absent pathology and 5 having severe pathology. Young n=5, AdV n=6, 1 mo post DSB n=4, 2 mo post DSB n=4, old n=3. Data shown are the mean values±s.e.m. P values were calculated using the Kruskal–Wallis test to AdV samples followed by a post hoc Dunn's test. *P<0.05, **P<0.01. #P value=0.06.