Table 1.
Mass spectrometric identification of proteins secreted by hASCs
| Protein (accession number) | SignalP* | Bone-related function | Protein (accession number) | SignalP | Bone-related function |
|---|---|---|---|---|---|
| Fibronectin (Q9UMK2) | 0.997 | Osteoblast survival and differentiation [20] | Collagen α1 (I) chain (Q9UML6) | 0.999 | Osteoblast maturation and differentiation [42] |
| Collagen α2 (I) chain (P08123) | 0.997 | Osteoblast maturation and differentiation [42] | Thrombospondin-2 (P35442) | 1.000 | Osteoblast differentiation [43] |
| Collagen α1 (III) chain (P02461) | 0.999 | Collagen α1 (VI) chain (P12109) | 1.000 | ||
| SR calcium ATPase 1 (O14983) | Thrombospondin-1 (P07996) | 0.994 | Osteoclast function [22] | ||
| α-actinin-1 (P12814) | Protein FAM40A (Q5VSL9) | ||||
| Periostin (Q15063) | 0.999 | Osteoblast proliferation and differentiation [19] | β ig-h3 (Q15582) | 1.000 | Osteoblast adhesion and differentiation [44] |
| Thrombospondin-5 (P49747) | 1.000 | Chondrocyte proliferation [45] | Matrix metalloproteinase 1 (P03956) | 1.000 | Osteoblast differentiation [46] |
| 72 kD type IV collagenase (P08253) | 1.000 | Bone cell growth and proliferation [47] | Plasminogen activator inhibitor 1 (P05121) | 0.999 | Bone mineralization and bone growth [48] |
| Albumin (P02768) | 1.000 | Moesin (P26038) | |||
| Galectin-3-binding protein (Q08380) | 1.000 | Protein disulfide-isomerase (P07237) | 1.000 | ||
| Fibulin-3 (Q12805) | 0.999 | Vimentin (P08670) | |||
| EF-1-alpha 1 (P68104) | Serpin A12 (Q8IW75) | 0.997 | |||
| Cathepsin L1 (P07711) | 0.999 | Bone resorption [49] | Cathepsin D (P07339) | 1.000 | Osteoblast calcification [50] |
| Glia-derived nexin (P07093) | 0.995 | Pentraxin-related protein PTX3 (P26022) | 1.000 | ||
| β-actin (P60709) | Decorin (P07585) | 1.000 | |||
| Follistatin-related protein 1 (Q12841) | 1.000 | Sulfhydryl oxidase 1 (O00391) | 1.000 | ||
| IGF-binding protein 7 (Q16270) | 0.998 | Cathepsin B (P07858) | 1.000 | ||
| Peptidyl-prolyl cis-trans isomerase B (P23284) | 0.863 | SPARC (P09486) | 1.000 | Osteoblast formation, maturation and survival [18] | |
| Lumican (P51884) | 1.000 | Bone formation [51] | Metalloproteinase inhibitor 2 (P16035) | 1.000 | Osteoblast differentiation [52] |
| Transgelin (Q01995) | Peroxiredoxin-1 (Q06830) | ||||
| Metalloproteinase inhibitor 1 (P01033) | Bone turnover [53] | Protein S100-A6 (P06703) | |||
| β2-microglobulin (P61769) | 1.000 | Osteoclast formation [21] |
*
SignalP indicates the probability score calculated with SignalP software.