Table 1.

PCR primer sequences, annealing temperatures and product sizes

Gene (Genbank access no.)	Primer sequence	Annealing temperature	Size (bp)
β-actin (NM_007393)	Sense: 5′-TGTTACCAACTGGGACGACA-3′ Antisense: 5′-GGGGTGTTGAAGGTCTCAAA-3′	Annealing: 57.5°C	165
NK1R (NM_009313)	Sense: 5′-GCTGCCCTTCCACATCTTCT-3′ Antisense: 5′-TTCCAGCCCCTCATAATCAC-3′	Annealing: 57.5°C	223
PPT-A (NM_009311)	Sense: 5′-CGCGATGCAGAACTACGAAA-3′ Antisense: 5′GCTTGGACAGCTCCTTCATC-3′	Annealing: 57.5°C	282