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Double-positive cells for DiI-acLDL uptake and FITC-UEA-1 binding on the substrates with varied stiffness under culture conditions of 20 ng/ml VEGF. (A) direct fluorescent staining; (B) comparisons of rates of double-positive cells; (C) comparisons of numbers of double-positive cells per HPF. Bars mean S.D. FITC-UEA-1: FITC-labelled ulex europaeus agglutinin I lectin; DiI-acLDL: DiI-labelled acetylated low-density lipoprotein. HPF: high power field. All P-values >0.05.
