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Immunofluorescence of CD117+ TCs, expanded during in vitro culture. A large fraction of cells (60%) showed proliferation. Proliferation was indicated by (A) the presence of Ki67 in the cell nucleus; and (B) endoglin/CD105 expression, at various intensities. (C) Pluripotency-associated transcription factor Oct4 stained positive in the considered cell population. (D) c-kit expression in TCs associated with an intermediate level of VEGF presence in the cytoplasm; magnification 400×.
