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. 2015 Mar 24;112(14):4447–4452. doi: 10.1073/pnas.1420363112

Fig. 1.

Fig. 1.

SMMHC-CreERT2/MyocdF/F mutant mice succumb from defects in the vasculature and visceral tissues. (A) Kaplan–Meier survival curves for tamoxifen-treated control MyocdF/F mice (n = 60) (black line) and SMMHC-CreERT2/MyocdF/F mice (red line) (n = 60). (B) Representative aorta harvested 4 mo following tamoxifen exposure of a MyocdF/F control mouse (Left) and SMMHC-CreERT2/MyocdF/F mutant mouse (Right). (C and D) H&E-stained section cut at the level of the ascending aorta harvested 4 mo following tamoxifen treatment of a MyocdF/F control mouse (C) and SMMHC-CreERT2/MyocdF/F mutant mouse (D). Arrows identify the aortic tunica media. Original magnification, 400×. (E–G) H&E-stained serial sections cut at the level of the ascending aorta in a tamoxifen-treated MyocdF/F control (E, 200×) and SMMHC-CreERT2/MyocdF/F mutant (F, 400×; G, 200×) mouse demonstrating aortic dissections with disruption of the tunica intima generating a false lumen (arrow, F) and arterial rupture (arrow, G). (H) Stomach harvested 4 mo following tamoxifen exposure harvested from a MyocdF/F control mouse (Left) and SMMHC-CreERT2/MyocdF/F mutant mouse (Right). (I and J) H&E-stained sections cut through the stomach of MyocdF/F control mouse (I) and SMMHC-CreERT2/MyocdF/F mutant mouse (J) with arrows demarcating the muscularis mucosa. Original magnification, 200×. (K) Small intestine harvested 4 mo following tamoxifen exposure harvested from a MyocdF/F control mouse (Left) and SMMHC-CreERT2/MyocdF/F mutant mouse (Right). (L and M) H&E-stained sections cut through the small intestine of MyocdF/F control mouse (L) and SMMHC-CreERT2/MyocdF/F mutant mouse (M) with arrows demarcating the muscularis mucosa. (N) Bladder harvested 4 mo following tamoxifen exposure harvested from a MyocdF/F control mouse (Left) and SMMHC-CreERT2/MyocdF/F mutant mouse (Right). (O and P) H&E-stained sections cut through the bladder of MyocdF/F control mouse (O) and SMMHC-CreERT2/MyocdF/F mutant mouse (P) with arrows demarcating the muscularis mucosa. L, M, O, and P, original magnification, 400×.