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. 2015 Mar 23;112(14):4465–4470. doi: 10.1073/pnas.1424989112

Table 1.

CO uptake observed for A. ehrlichii MLHE-1 incubated with varied electron acceptors (suboxic, 0.2% O2; anoxic, +5 mM nitrate) or magnesium perchlorate (50 mM) or magnesium sulfate (500 mM)

Uptake units Treatments
Oxic Suboxic Anoxic
Rate, nmol−1·mgdw−1·h−1 1.61 ± 0.39 1.49 ± 0.26 0.23 ± 0.03
Control Mg(ClO4)2 MgSO4
Rate, nmol−1·mgdw−1·h−1 0.49 ± 0.07 0.52 ± 0.05 0.63 ± 0.09
Control Vacuum control 10 mbar
Rate constant, h−1 0.91 ± 0.04 1.25 ± 0.31 0.67 ± 0.16

Uptake rate constants (h−1 ± 1 SE, n = 3) were observed for ambient and low-pressure incubations. Rates for varied electron acceptor and magnesium salt availability [nmol (mg cell dry weight)−1⋅h−1 ± 1 SE, n = 3] were determined for cells incubated with initial headspace CO concentrations of 8 ppm and were based on first-order uptake rate constants. Rate constants for varied pressure incubations were obtained for treatments initiated at 6 ppm CO and 1 atm pressure (controls), for treatments with 6 ppm CO and 1 atm pressure reestablished after reducing pressure to 10 mbar (vacuum controls), and for treatments with 600 ppm CO at 10 mbar pressure. mgdw, mg cell dry weight.