Fig. 2.

Effect of P2 receptor blocker suramin on intracellular Ca²⁺ induced by ATP in 16HBE cells. Intracellular Ca²⁺ measurement in single 16HBE cells using Fluo 3/AM as a probe. (A) The addition of ATP (10 μM) significantly increased the intracellular Ca²⁺, which was inhibited in the presence of the P2-R antagonist, suramin (100 μM); the addition of 1 μM Thapsigargin (Tg, an inducer of stored Ca²⁺ release) was done to check the activity of cells. (B) A summary of the effects of suramin on ATP-induced calcium elevation. Data are presented as mean of fluorescence intensity ± SEM (n = 4) and are based on four independent experiments. **P < 0.01.