Fig. 3.

Effect of T. gondii on intracellular Ca²⁺ induced by ATP in 16HBE cells. Intracellular Ca²⁺ measurement in single 16HBE cells using Fluo 3/AM as a probe. (A) Addition of ATP (10 μM) significantly increased the intracellular Ca²⁺ inhibited after T. gondii pretreatment for 3 h or 6 h. (B) A summary of the effects of T. gondii on ATP-induced calcium elevation. Data are presented as mean fluorescence intensity ± SEM (n = 4) and are based on four independent experiments. ***P < 0.001; NS, P > 0.05.