Table 3.

Functional mutants tested

Gene	RCH at 25 °C		RCH at 18 °C		DACC
	Score	P value	Score	P value	Score	P value
Background	0.48		0.90		0.74
Atg7	0.25	0.017	0.87	0.27	NA	NA
CG8398	0.31	0.32	0.49	<0.001	NA	NA
CG32111	0.38	0.023	0.68	0.0077	0.78	0.62
CG33275	0.56	0.12	0.82	0.21	0.70	0.56
CG33275	0.20	0.22	0.85	0.30	0.86	0.058
dpr5	−0.14	0.16	0.56	<0.001	0.52	0.016
Eip74EF	0.54	0.49	0.71	0.0056	0.00	0.32
Fili	0.54	0.51	0.48	<0.001	0.59	0.10
Glu-RI	0.20	0.17	0.81	0.16	0.78	0.63
Prosap	0.48	0.98	NA	NA	NA	NA
Px	0.34	0.42	0.65	0.0065	0.64	0.16
Syt12	0.37	0.23	NA	NA	0.55	0.016
VGlut	0.43	0.55	0.67	<0.001	0.40	<0.001

We were unable to test some mutants because of poor growth and viability when developmentally acclimated at 18 °C. A significant interaction term between genotype and environment (cold stress with and without acclimation) indicated that the mutant differed in acclimation capacity compared with the genetic background control (w1118), represented here under the P value column. Boldface type indicates significance at P < 0.05. The score represents the difference between survivorship following acute stress (at −6 °C for 1 h) for RCH at 25 °C and DACC and at −8 °C for 1 h for RCH after DACC at 18 °C and survivorship after acclimation for 2 h at 4 °C followed by the acute stress. For survivorship proportions used to calculate score, see Dataset S2.