Table 1.

Primers used in this study

DOI: http://dx.doi.org/10.7554/eLife.04872.019

hPPP1R15A truncation or point mutation
1–615	For	CCTGCTGCCCGGGCCAGAGCCTGAGCACGCCTCAGGAACC
	Rev	GGTTCCTGAGGCGTGCTCAGGCTCTGGCCCGGGCAGCAGG
1–620	For	CCTGGGCACGCCTCAGGTAGCCACCTTTAGCC
	Rev	GGCTAAAGGTGGCTACCTGAGGCGTGCCCAGG
501–654	For	TAAGCTAGCACCATGGAAGCTGAGCCC
	Rev	GTCGCGGCCGCTTTACTTGTACAGCTC
V556E	For	CTAAAGGCCAGAAAGGAGCGCTTCTCCGAGAAGGTCACTG
	Rev	CAGTGACCTTCTCGGAGAAGCGCTCCTTTCTGGCCTTTAG
W616A	For	CCGGGCCAGAGCCGCGGCACGCCTCAGGAA
	Rev	TTCCTGAGGCGTGCCGCGGCTCTGGCCCGG
R618A	For	GCCAGAGCCTGGGCAGCCCTCAGGAACCCA
	Rev	TGGGTTCCTGAGGGCTGCCCAGGCTCTGGC
L619A	For	AGCCTGGGCACGCGCCAGGAACCCACCTTT
	Rev	AAAGGTGGGTTCCTGGCGCGTGCCCAGGCT
R620A	For	CCTGGGCACGCCTCGCGAACCCACCTTTAG
	Rev	CTAAAGGTGGGTTCGCGAGGCGTGCCCAGG
W616A/L619A	For	CGGGCCAGAGCCGCGGCACGCGCCAGGAACCC
	Rev	GGGTTCCTGGCGCGTGCCGCGGCTCTGGCCCG
mDia2 fusion	For	AATCCCGGATCCGTGCCTTGCCACCTGGTACA
	Rev	AGCTCGCTCGAGTTATAAAGCTCGTAATCTTGCCAG
PPP1R15B fusion
GFP fusion	For	AGATTAGATCTGCCACCATGGAGCCGGGGACAGG
	Rev	GATCGTCGACACATTGCTTGAGAACATTAAGTCC
dPPP1R15 truncation
1–307	For	CCAGTTCACCGAGATCGTTAGTACCAAGCTCGATTCTTGCACG
	Rev	CGTGCAAGAATCGAGCTTGGTACTAACGATCTCGGTGAACTGG
1–312	For	CGTGTCTACCAAGCTCGATAGTTGCACGAGGACGAGC
	Rev	GCTCGTCCTCGTGCAACTATCGAGCTTGGTAGACACG