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. 2015 Apr 13;5:9676. doi: 10.1038/srep09676

Figure 3. Muscle-specific Raptor knockdown flies die around pupal stage ~12–13 but pre-eclosure.

Figure 3

The graph depicts the birthrates of the progeny from crosses of homozygous GAL4 driver lines with homozygous Raptor shRNA transgenic flies or a control TRiP line. Panels a, c and f indicate Mef2-GAL4 driven knockdowns while b, d and g indicate c179-GAL4 mediated knockdowns. (a) and (b) show the total number of flies eclosed; (c) and (d) indicate the total number of pupae after 20 days, and (f) and (g) show the percentage of dead pupae. Panel (e) shows a representative example of dead flies, still within their pupal cases for Mef2-GAL4 > UAS-Raptor-shRNA #1. Asterisks indicate p < 0.05 by ANOVA followed by Dunnett's test (b, c and d) or Kruskal-Wallis tests then Wilcoxon rank-sum tests followed by an adjustment for multiple comparisons (a, f and g). Each of these analyses describe the average 5–9 independent crosses, with error bars indicating standard error of the mean between replicate crosses.