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. 2015 Apr 13;5:9676. doi: 10.1038/srep09676

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(a) Flies with Mef2-GAL4 driven knockdown of the control shRNA or Raptor shRNA #1 were examined from pupal stage 10 till after expected eclosure. By day 14 after the cross, control flies had completely eclosed but Raptor knockdown flies remained inside the pupal cases. (b) On day 10, (approximately pupal stage 12) pupal cases were cut open at the operculum to assist eclosure, then after 4 days, climbing assays were performed on escaping flies. The Mef2-GAL4 > Raptor shRNA flies that eclosed with assistance had significantly impaired climbing. Asterisk indicates p < 0.005 by Wilcoxon Rank Sum test. Data represents mean +/− standard error of the mean.