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. Author manuscript; available in PMC: 2015 Apr 13.
Published in final edited form as: Mol Microbiol. 2010 Feb 18;75(6):1563–1576. doi: 10.1111/j.1365-2958.2010.07078.x

Fig. 6. Localization of FliG1 in B. burgdorferi.

Fig. 6

A. Constructing a vector that expresses the N-terminal GFP-fusion proteins. The flgB promoter element was fused to gfp with a five-glycine linker, and SphI cloning site was engineered at the 3′ end of gfp. A target gene, such as fliG1, will be fused to gfp at the SphI site. A T7 transcription terminator was inserted.

B. Detection of GFP–FliG1 fusion protein using Western blot. A monoclonal antibody against GFP (Invitrogen) was used to detect GFP and GFP–FliG1. GFP is about 27 kDa and GFP–FliG1 is about 75 kDa.

C. The fluorescence micrograph (40×) of the fliG1 mutant that was transformed with the plasmid pFBgfp/T7t, which only expresses GFP.

D. The fluorescence micrograph (40×) of the fliG1 mutant that was transformed with the plasmid pFBgfp–fliG1/T7t, which expresses GFP–FliG1 fusion protein. The brighter ends represent the location of GFP–FliG1.