Fig. 7.

Localization of FliG2 in B. burgdorferi by immunofluorescence microscopy. The wild-type and the fliG2− mutant cells were fixed with methanol, stained with anti-FliG2 and counterstained with anti-rat Texas red-coupled antibody, as described in Experimental procedures. Photo (DIC) and fluorescent (TRITC) micrographs (100×) were, respectively, taken under a Zeiss Axioimager Z1 Axiophot microscope, and merged. For each strain, more than 50 cells were examined.