Figure 4.
Expression analysis of the internal control genes evaluated in this study during different phases of lung development. (A) Variable cycle threshold (CT) for the different reference genes over mouse lung development. CT values were determined by qRT-PCR in triplicates for biological duplicates (n = 6) at the indicated stages of lung development. The CT values were normalized to total RNA quantity used for the RT reaction. Data are represented as mean CT ± SD; (B) CT distribution of each putative reference gene among all mouse lung development stages analysed here. CT values were determined as in A. For each reference gene, the mean CT for each stage (points) and the mean CT for all stages (line) were plotted to represent CT variation between stages of lung development.