Table 4.

Pairwise variation coefficient calculated for sequential normalization factors for each of the five experimental groups. Pairwise variation coefficient of the most stable genes was calculated followed by sequential inclusion of less stable genes. Group 1, branching morphogenesis (E11.5–E16.5); Group 2, differentiation, canalicular and saccular (E16.5–P5); Group 3, prenatal development (E11.5–E18.5); Group 4, postnatal development (P1–P28); Group 5, all stages.

Group	V2/3	V3/4	V4/5
Group 1	Tuba1a, Gapdh	Tuba1a, Gapdh, Actb	Tuba1a, Gapdh, Rn18S
	0.091462	0.179303	0.296517
Group 2	Tuba1a, Actb	Tuba1a, Gapdh, Actb	Tuba1a, Gapdh, Rn18S
	0.173682	0.12009	0.30073
Group 3	Tuba1a, Actb	Tuba1a, Gapdh, Actb	Tuba1a, Gapdh, Rn18S
	0.094428	0.786888	0.978725
Group 4	Tuba1a, Gapdh	Tuba1a, Gapdh, Actb	Tuba1a, Gapdh, Rn18S
	0.234143	0.343936	0.333048
Group 5	Tuba1a, Actb	Tuba1a, Gapdh, Actb	Tuba1a, Gapdh, Rn18S
	0.29673	1.143188	1.018239