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. 2015 Mar 2;16(3):4666–4681. doi: 10.3390/ijms16034666

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© 2015 by the authors; licensee MDPI, Basel, Switzerland.

This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/4.0/).

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Histological analyses of hyaluronan-DPSCs bone graft one month after rat calvarial defect repair. (a) Azan Mallory staining of collagen matrix (yellow arrow), blood vessels (red arrow) and hyaluronan fibers (white circles); (b) Masson’s trichrome staining of collagen matrix (yellow arrow), blood vessels (red arrow) and hyaluronan fibers (purple circles); (c) Azan Mallory and (d) Masson trichrome staining of hyaluronan bone graft without cells; and (e) Gene expression profile of dentin ECM-related markers (dentin sialophosphoprotein (DSPP), bone morphogenetic protein 2 (BMP2), BMP3), bone ECM-related markers (COL1A1, COL3A1, cathepsin B (CTSB), cathepsin D (CTSD)) and other bone markers (fibroblast growth factor 8 (FGF8), msh homeobox 1 (MSX1), tumor necrosis factor receptor superfamily (TNFRSF11A)) in the hyaluronan-DPSC bone graft (black bars) compared to the hyaluronan scaffold alone (white bars). Significant differences between hyaluronan-DPSCs bone graft and hyaluronan scaffold alone (** p < 0.01; *** p < 0.001); R: relative expression.

Histological analyses of hyaluronan-DPSCs bone graft one month after rat calvarial defect repair. (a) Azan Mallory staining of collagen matrix (yellow arrow), blood vessels (red arrow) and hyaluronan fibers (white circles); (b) Masson’s trichrome staining of collagen matrix (yellow arrow), blood vessels (red arrow) and hyaluronan fibers (purple circles); (c) Azan Mallory and (d) Masson trichrome staining of hyaluronan bone graft without cells; and (e) Gene expression profile of dentin ECM-related markers (dentin sialophosphoprotein (DSPP), bone morphogenetic protein 2 (BMP2), BMP3), bone ECM-related markers (COL1A1, COL3A1, cathepsin B (CTSB), cathepsin D (CTSD)) and other bone markers (fibroblast growth factor 8 (FGF8), msh homeobox 1 (MSX1), tumor necrosis factor receptor superfamily (TNFRSF11A)) in the hyaluronan-DPSC bone graft (black bars) compared to the hyaluronan scaffold alone (white bars). Significant differences between hyaluronan-DPSCs bone graft and hyaluronan scaffold alone (** p < 0.01; *** p < 0.001); R: relative expression.