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(a) Duplexed LAMP reactions with Rs R3B2-specific primer set rk2208.1 (blue lines) and Rs species-level primer set egl62 (red lines) conducted with template DNA from Rs R3B2 strain UW551; (b) Quantitative relationship of observed threshold times for rk2208.1 probes () and egl62 probes () with UW551 DNA in duplexed reaction.
