Table 1.
Effective doubling times for different reactions and reaction conditions.
| Reaction | Single | Duplex | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Template DNA | Salmonella | phage λ | Rs R3B2 | Rs R3B2 | Salmonella and phage λ | Rs R3B2 (UW551) | Rs (GMI1000) | |||
| Primer Set | Se | λ-phage | egl62 | rk2208.1 | Se and λ-phage | egl62 and rk2208.1 | egl62 and rk2208.1 | |||
| Probes | Se | λ | egl62 | rk2208.1 | Se | λ | egl62 | rk2208.1 | egl62 | rk2208.1 |
| τD a | 28.1 ± 2.9 | 29.2 ± 1.6 | 47.6 ± 7.6 | 28.9 ± 3.4 | 39.1 ± 6.7 | - | 31.9 ± 3.4 | 37.9 ± 2.1 | 36.1 ± 3.1 | - |
| LOD b | 98 | 1000 | 86 | 82 | 9800 | - | 860 | 820 | 860 | - |
a Doubling time for given LAMP reaction (in seconds). b Detection limit (in genomic equivalents of template DNA per reaction).