Table 2.
Loop-mediated isothermal AMPlification (LAMP) primer and Assimilating Probe sequences.
| - | Nucleotide Sequence (5'→3') |
|---|---|
| Se primer set: designed to detect Salmonella enterica | |
| Se F3 | GGCGA TATTG GTGTT TATGG GG |
| Se B3 | TGAAC CTTTG GTAAT AACGA TAAAC TG |
| Se FIP 1 | CTGGT ACTGA TCGAT AATGC CAAGT TTTTC AACGT TTCCT GCGG |
| Se BIP 1 | GATGC CGGTG AAATT ATCGC ACAAA ACCCA CCGCC AGG |
| Se loopF | GACGA AAGAG CGTGG TAATT AAC |
| Se loopB | GGGCA ATTCG TTATT GGCG |
| λ primer set: designed to detect Enterobacterio phage λ | |
| λ-phage F3 | GGCTT GGCTC TGCTA ACACG TT |
| λ-phage B3 | GGACG TTTGT AATGT CCGCT CC |
| λ-phage FIP 1 | CAGCC AGCCG CAGCA CGTTC GCTCA TAGGA GATAT GGTAG AGCCG C |
| λ-phage BIP 1 | GAGAG AATTT GTACC ACCTC CCACC GGGCA CATAG CAGTC CTAGG GACAG T |
| λ-phage loopF | CTGCA TACGA CGTGT CT |
| λ-phage loopB | ACCAT CTATG ACTGT ACGCC |
| egl62 primer set: designed to detect Ralstonia solanacearum | |
| egl62 F3 | CTGGA ACCAG AACTG GTACG |
| egl62 B3 | ATAGC CGTTG CTGCG C |
| egl62 FIP 1 | TGGTG CACCT CGAAG ACGAG GTCCG AACGG CACCG TCATG |
| egl62 BIP 1 | CGATT CGTCC GGCCA GTCGC CAGTT GGTGA AGTCC TGC |
| egl62 loopF | CCGGG TCATT GATGC CCTT |
| rk2208.1 primer set: designed to detect Ralstonia solanacearum race 3 biovar 2 strains | |
| rk2208.1 F3 | GAGAG ACATG TCCGA TTCCG |
| rk2208.1 B3 | GCCGA TGTCA TCAAG CTCAA |
| rk2208.1 FIP 1 | TGTGA CTTCC ACGTC AAGCG TTGCA ATCAC CGACT TCCTC A |
| rk2208.1 BIP 1 | GCGAG AAGCC CGTGT GCTTG TCACG ATTTT CGGCC AGTT |
| rk2208.1 loopB | AGAGC TTTTC GCCAA TCGAC T |
| Assimilating probes | |
| Se F strand loopF 3 | FAM 2—ACGCT GAGGA CCCGG ATGCG AATGC GGATG CGGAT GCCGA GACGA AAGAG CGTGG TAATT AAC |
| Se F strand loopB 3 | FAM 2—ACGCT GAGGA CCCGG ATGCG AATGC GGATG CGGAT GCCGA GGGCA ATTCG TTATT GGCG |
| λ-phage F strand loopF 3 | TAMRA 4—ACGCT GAGGA CCCGG ATGCG AATGC GGATG CGGAT GCCGA CTGCA TACGA CGTGT CT |
| egl62 F strand loopF 3 | TAMRA 4—ACGCT GAGGA CCCGG ATGCG AATGC GGATG CGGAT GCCGA CCGGG TCATT GATGC CCTT |
| rk2208.1 F strand loopB 3 | FAM 2—ACGCT GAGGA CCCGG ATGCG AATGC GGATG CGGAT GCCGA AGAGC TTTTC GCCAA TCGAC T |
| Quench strand | TCGGC ATCCG CATCC GCATT CGCAT CCGGG TCCTC AGCGT—Q 5 |
1 Underlined text represents F2/B2 sequence in FIP/BIP primer; 2 FAM: 6-carboxyfluorescein; 3 Bold text represents loop primer sequence used in Assimilating Probes; 4 TAMRA: carboxytetramethylrhodamine; 5 Q: Iowa Black Quencher-1, Integrated DNA Technologies, Coralville, IA, USA.