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. 2015 Mar 9;16(3):5363–5374. doi: 10.3390/ijms16035363

Figure 3.

Figure 3

SiRNA-mediated knockdown of Tspan8 or integrin α3 inhibits U251MG cell migration. In vitro migration of U251MG cells transfected with or without indicated siRNA (100 nM, 48 h) was tested by “transwell” assay described (A, 18 h); the number of migrated cells was counted and normalized to the control U251MG cells (“NO siRNA”) (B); Expression of Tspan8, integrin α3 and GAPDH in above U251MG cells was shown in (C); Above U251MG cells were also subjected to MTT cell viability assay, and OD values were normalized to the control U251MG cells (D, 18 h). Magnification: 1:100 (A). Data were presented as mean ± SD. * p < 0.05 vs. control U251MG cells.