Figure 5.

Fluorescence lifetime of the donor ECFP in transfected HeLa multicellular spheroids prior to and subsequent to application of staurosporine (2 µM, 2.5 h). Medians ± median absolute deviations (MADs) of six cell layers (from three spheroids) each selected by light sheet based fluorescence microscopy. Excitation wavelength: 391 nm; fluorescence detected at 450–490 nm).