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. 2015 Mar 10;16(3):5434–5451. doi: 10.3390/ijms16035434

Figure 1.

Figure 1

siRNA technology reduces suppressor of cytokine signaling 3 (SOCS3) expression in eosinophils from asthmatic patients. (A) SOCS3, SOCS1 and SOCS5 mRNA relative levels were measured by quantitative PCR in eosinophils purified from asthmatic patients (n = 36); (B) After SOCS3 siRNA incubation, quantitative-PCR results showed significant down-regulation of SOCS3 mRNA levels and no alteration of SOCS5 or SOCS1, as well as decreased protein expression level of SOCS3 after SOCS3 siRNA treatment was detected by Western blot analysis and further quantified by densitometry (C) or immnunofluoresce (D) as is depicted on a representative picture of an eosinophil incubated with non-target (NT)siRNA (left panel) or SOCS3 siRNA (right panel), after staining with anti-SOCS3 antibody labeled with Alexa 647 fluorochrome. The results were expressed as mean ± SD, (n = 6–8). * Denotes statistical significance (p < 0.05); ** denotes obvious statistical significance (p < 0.01).