Expression of 22 genes in control and drought-treated roots of tobacco. (A) The levels of 22 genes detected by Solexa sequencing in three Digital Gene Expression (DGE) libraries (NCK, N6H and N48H); (B) The levels of these 22 genes detected by quantitative Real-Time PCR (qRT-PCR) in NCK, N6H and N48H (normalized to actin; n = 3). The green, purple and blue bars in graph B depicted the qRT-PCR relative expression level ± standard error of three replicates for each gene in NCK, N6H and N48H, respectively. NCK, N6H and N48H represented three samples of tobacco roots at 0 h (control, without drought-treatment) and two drought-treatment time-points (6 and 48 h), respectively.