Skip to main content
. 2015 Mar 20;16(3):6447–6463. doi: 10.3390/ijms16036447

Figure 1.

Figure 1

Transcriptional upregulation of specific ligands of the EGF family can be suppressed by inhibition of mAChRs, EGFR and MAP kinase signaling. Transcript levels of the EGF family ligands HB-EGF (A–C), TGFα (D–F), AREG (G–I) and EREG (J–L) were measured by qPCR in cells stimulated for 2 h with 100 µM CCh and treated with inhibitors of mAChRs (A,D,G,J: Atropin), EGFR (B,E,H,K: PD 153035) or MEK1/2 (C,F,I,L: U0126). Bars represent the mean ± SD of three independent experiments. Statistical analysis was performed with one-way or two-way ANOVA; *,# p < 0.05, **,## p < 0.01, ***,### p < 0.001. * refers to comparison to unstimulated control, # to stimulated sample without inhibitor.