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Egr1 transcription is stimulated by CCh and repressed by inhibition of mAChRs (A), EGFR (B) and MAP kinases (C). Transcriptional induction of Egr1 was measured by qPCR as stated in the legend of Figure 2. Bars represent the mean ± SD of three independent experiments. Statistical analysis was performed with one-way or two-way ANOVA; * p < 0.05, **,## p < 0.01, ***,### p < 0.001. * refers to comparison to unstimulated control, # to stimulated sample without inhibitor.
