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Cholinergic stimulation of HaCaT cells results in increased flotillin expression. HaCaT cells were stimulated with 100 µM CCh as indicated and the mRNA (A) and protein (B) levels of flotillins were determined. Bars in (A) represent the mean of 3 independent experiments. Statistical analysis was performed with one-way ANOVA; * p < 0.05, ** p < 0.01; (B) shows a representative Western blot for flotillins upon CCh stimulation.
