Table 1.
Gene | Primer Forward | Primer Reverse | TAnnealing (°C) |
---|---|---|---|
cFos | TGGTGAAGACCGTGTCAGGAG | TGATCTGTCTCCGCTTGGAGTG | 60 |
Dusp1 | GGAGGACAACCAGGCAGAC | AGGTAAGCAAGGCAGATGGTGG | 60 |
Egr1 | TTCAACCCTCAGGCGGACAC | GTCTCCACCAGCACCTTCTCGT | 60 |
HPRT | GCAGTCCCAGGGTGCGTG | GGCCTCCCATCTCCTTCAT | 60 |
Ywhaz | AGGTTGCCGCTGGTGATGAC | GGCCAGACCCAGTCTGATAGGA | 60 |
Rpl13a | CCTGGAGGAGAAGAGGAAAGAGA | TTGAGGACCTCTGTGTATTTGTCAA | 60 |
BTC | GCGGAAAGGCCACTTCTCTAGG | TCATCACAGACACAGGAGGGCG | 60 |
EGF | GCAGCTTCAGGACCACAACCA | AAACCATTCCCATCTGCTGGCT | 60 |
EPGN | ACTCCAGAGGCTGACACAGGAC | GGAGTTTCGCTCTTGTCACCCA | 60 |
HB-EGF | GTCTGTCTGCTGGTCATCGTGG | CCCAGCCGATTCCTTGAGCA | 60 |
EREG | GGTTTCCATCTTCTACAGGCA | TGTCTTCTGTCTGAACTAAAGCTG | 60 |
AREG | GATACTCGGCTCAGGCCATTATGC | TCAAATCCATCAGCACTGTGGTCC | 60 |
TGFα | GCTGATACACTGCTGCCAGGTC | AGCAAGCGGTTCTTCCCTTCAG | 60 |
ADAM17 | TCGAGGGTGGATGAAGGAGAAGAG | GGACTGTTCCTGTCACTGCACTG | 62 |
ADAM10 | TCCACAGCCCATTCAGCAA | GCGTCTCATGTGTCCCATTTG | 60 |
ADAM8 | CACCAAAGCAGGTCATCAAGCCA | GCAACCTTTGGGCCAACAGCAC | 62 |
MMP-1 | CTGCCAAATGGGCTTGAAGCTGC | AGTTCTAGGGAAGCCAAAGGAGCT | 62 |
MMP-2 | CCTGACATTGACCTTGGCACCG | GTCCGCCAAATGAACCGGTCCT | 62 |
MMP-3 | ATTCAGTCCCTCTATGGACCTCCC | ACAGCATCAAAGGACAAAGCAGGA | 58 |
MMP-7 | TGGGAACAGGCTCAGGACTATCTC | TGACGCGGGAGTTTAACATTCCAG | 62 |
MMP-9 | TTCTTCTCTGGGCGCCAGGT | CCGCTGAACAGCAGCATCTTCC | 58 |