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. 2015 Mar 20;16(3):6447–6463. doi: 10.3390/ijms16036447

Table 1.

Primer sequences and annealing temperatures for the qPCR primers used in this study. All sequences are given in 5'–3' direction.

Gene Primer Forward Primer Reverse TAnnealing (°C)
cFos TGGTGAAGACCGTGTCAGGAG TGATCTGTCTCCGCTTGGAGTG 60
Dusp1 GGAGGACAACCAGGCAGAC AGGTAAGCAAGGCAGATGGTGG 60
Egr1 TTCAACCCTCAGGCGGACAC GTCTCCACCAGCACCTTCTCGT 60
HPRT GCAGTCCCAGGGTGCGTG GGCCTCCCATCTCCTTCAT 60
Ywhaz AGGTTGCCGCTGGTGATGAC GGCCAGACCCAGTCTGATAGGA 60
Rpl13a CCTGGAGGAGAAGAGGAAAGAGA TTGAGGACCTCTGTGTATTTGTCAA 60
BTC GCGGAAAGGCCACTTCTCTAGG TCATCACAGACACAGGAGGGCG 60
EGF GCAGCTTCAGGACCACAACCA AAACCATTCCCATCTGCTGGCT 60
EPGN ACTCCAGAGGCTGACACAGGAC GGAGTTTCGCTCTTGTCACCCA 60
HB-EGF GTCTGTCTGCTGGTCATCGTGG CCCAGCCGATTCCTTGAGCA 60
EREG GGTTTCCATCTTCTACAGGCA TGTCTTCTGTCTGAACTAAAGCTG 60
AREG GATACTCGGCTCAGGCCATTATGC TCAAATCCATCAGCACTGTGGTCC 60
TGFα GCTGATACACTGCTGCCAGGTC AGCAAGCGGTTCTTCCCTTCAG 60
ADAM17 TCGAGGGTGGATGAAGGAGAAGAG GGACTGTTCCTGTCACTGCACTG 62
ADAM10 TCCACAGCCCATTCAGCAA GCGTCTCATGTGTCCCATTTG 60
ADAM8 CACCAAAGCAGGTCATCAAGCCA GCAACCTTTGGGCCAACAGCAC 62
MMP-1 CTGCCAAATGGGCTTGAAGCTGC AGTTCTAGGGAAGCCAAAGGAGCT 62
MMP-2 CCTGACATTGACCTTGGCACCG GTCCGCCAAATGAACCGGTCCT 62
MMP-3 ATTCAGTCCCTCTATGGACCTCCC ACAGCATCAAAGGACAAAGCAGGA 58
MMP-7 TGGGAACAGGCTCAGGACTATCTC TGACGCGGGAGTTTAACATTCCAG 62
MMP-9 TTCTTCTCTGGGCGCCAGGT CCGCTGAACAGCAGCATCTTCC 58