Figure 3.

Coexpression of cyclic peptides bearing various ncAA initiators and ncAA elongators. (a) The initiation table (tableⁱⁿⁱ, blue) designates multiple initiators while the elongation table (table^elon, red) designates various ncAAs as well as cognate amino acids. Some of codon triplets are dual sense, i.e., designating different amino acids in tableⁱⁿⁱ and table^elon; (b) Schematic illustration of the coexpression. Blue and red tRNAs indicate initiator tRNAs^fMet and elongator tRNAs^AsnE2, respectively. The chloroacetylated ncAA initiators react post-translationally with a downstream Cys residue to form macrocyclic structures.