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. 2015 Apr 11;15:254. doi: 10.1186/s12885-015-1292-z

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© Li et al.; licensee BioMed Central. 2015

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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GF affected Bcl-2 family, MAPKs, and androgen receptor (AR). LNCaP cells were treated with 10 μM GF for 6, 12 and 24 h, respectively. (A) Western blot showed the expression of Bcl-2, Bax and Bcl-xL in LNCaP cells treated with 10 μM GF with or without FBS. (B) The ratio of Bcl-2/Bax protein expression. (C) Relative Bcl-2 and PUMA mRNA level (compared with GAPDH) was analyzed by quantitative RT-PCR. LNCaP cells were treated for 0 h as control. Cells were treated with GF (10 μM) in serum-free medium or serum medium for 6 h. * indicates a significant difference from the controls. p < 0.05. (D) The protein expression of MAPKs and AR, phosphorylated ERK and phosphorylated ERK in LNCaP cells treated with 10 μM GF with or without FBS.