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Peripheral immune responses against sporozoites. Mice were immunized with three doses of RAS each with 10,000 sporozoites at an interval of two weeks. Geometric mean titers (GMT) of anti-sporozoite antibody were determined by immunofluorescence assay (A) and CD8+ T cells in PBMCs were analyzed by flow cytometry (B) 7 days after the final immunization. The results are expressed as means ± SD. N=8, *p<0.05, **p<0.01, and ***p<0.001.
