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. Author manuscript; available in PMC: 2015 Apr 13.
Published in final edited form as: Oncogene. 2005 Jan 20;24(4):616–626. doi: 10.1038/sj.onc.1208125

Figure 1.

Figure 1

EGFR inhibitors induce apoptosis in human melanomas. (a) Cell surface EGFR expression on melanocytes and human melanomas was determined by staining with PE-conjugated anti-human EGFR monoclonal antibody and flow cytometry. Filled histograms represent nonspecific staining with mouse Ig-PE. Mean fluorescent intensity (MFI) is indicated. WM35 cells, as well as HHMSX, FEMX, LOX and OM431, are EGFR negative. (b) Dose–response induction of apoptosis by AG1478 in two melanoma lines, WM793 and WM9. (c) Cell-cycle apoptosis analysis of human melanocytes and melanoma cells treated with AG1478 or PD153035 (20 μM) for 24 h. Cells were stained with PI and percentage of cells with hypodiploid content of DNA was determined by flow cytometry