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. Author manuscript; available in PMC: 2015 Apr 13.
Published in final edited form as: Oncogene. 2005 Jan 20;24(4):616–626. doi: 10.1038/sj.onc.1208125

Figure 4.

Figure 4

Regulation of EGFR-mediated signaling in WM9 melanoma cells. (a) Western blot analysis of total and phospho-EGFR, phospho-ERK, phospho-p38 and phospho-AKT levels following AG1478 (20 μM), arsenite (10 μM) or combined treatment. Induction of HO-1 was used as an indication of arsenite-induced oxidative stress. β-Actin levelswere used asan internal control. (b) PARP cleavage wasdetermined by Western blot analysis 18 h after treatment with AG1478 (20 μM), PD153035 (20 μM) or arsenite (10 μM). (c and d) Overexpression of AKTmyr protectsWM9 melanoma cells from apoptosis induced by LY294002 (50 μM) or AG1478 (20 μM)