FIG. 5.

Cyclic stretch for 30 min post-transfection is sufficient for maximal enhancement of gene transfer. A549 cells were transfected with DNA using liposomes (A) or electroporation (B) and then stretched for 30 min and grown statically for 23.5 hours or stretched for 24 hours. Luciferase expression was normalized relative to expression seen in statically grown transfected cells. In these experiments, the absolute level of transfection was 10-fold greater in lipoplex-transfected cells. *, p < 0.01 versus 0 time. No statistical difference was observed between 0.5 and 24 hours for either method.