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. 2015 Apr 13;5:9650. doi: 10.1038/srep09650

Figure 6. Targeted downregulation of HuR plays an important role in miR-191 mediated TGFβ2 induction in hypoxic microenvironment.

Figure 6

(a). Effect of HuR on the level of TGFβ2 transcript in hypoxic microenvironment. Overexpression of HuR led to reduction in level of TGFβ2 mRNA while Silencing the HuR (esiHuR) led to significant induction in level of TGFβ2 mRNA. (b). Interaction profile of HuR protein with TGFβ2 3′UTR. The cloned 3′UTR region of TGFβ2 encompassing the binding sites of miR-191 and HuR is highlighted. The region was considered for determining the combined effect of both miR-191 and HuR in the regulation of TGFβ2. (c). qRT-PCR data showing regulation of TGFβ2 levels in MCF7 cells in response to overexpression of miR-191 or HuR or both. (d, e). Graph showing luciferase activity of TGFβ2-wild type (d) or mutated 3′UTR (e) in response to overexpression of miR-191 or HuR or both in MCF7 cells. Results show that miR-191 mediated induction of TGFβ2 was partially reduced in presence of HuR overexpression. The graphical data points in a & c-e represent mean ± S.D of at least three independent experiments. (*P < 0.05, **P < 0.01, *^P > 0.05 < 0.1). Error bars denote ± SD.